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Biological samples require a hydrated environment to maintain their structure and retain dynamic behavior.  With Protochips’ machine-vision powered suite of in-situ TEM tools you can utilize the electron microscope as a real-time laboratory to study delicate, low contrast systems at a resolution of a few nanometers.

Poseidon AX: Liquid, electrochemical and liquid heating in situ studies

Study nanoscale dynamics, interactions, and processes of virus, cells, proteins, micelles, and other biological and biomimetic materials while maintaining a physiologically relevant environment.

Lin, M. et al. (2024) 19, 646–651

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Featured Papers

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What have our users done in the field? Read our summaries here!

Videos

Watch dynamic behavior of real samples in situ.

This movie shows a series of images that demonstrates the tilt range of Poseidon Select. Images of aggregated 30 nm gold nanoparticles in 1.5 μm of water were collected in 5° increments, over a total angle of -30° to +30°. Data was collected using a Philips/FEI CM300FEG TEM equipped with a GIF energy filter at 300 kV.

Poseidon Select Tilt

This movie shows a series of images that demonstrates the tilt range of Poseidon Select. Images of aggregated 30 nm gold nanoparticles in 1.5 μm of water were collected in 5° increments, over a total angle of -30° to +30°. Data was collected using a Philips/FEI CM300FEG TEM equipped with a GIF energy filter at 300 kV.

Electron beam-induced growth of calcite nanoparticles in situ in the presence of AP7, a nacre protein that is found in abalone shellfish. The calcite particles were grown in the spatially confined conditions of the in-situ liquid cell in a liquid layer of 500 nm. During growth both a stable protein mediated scaffold-type structure, and an unstable crystalline structure which re-dissolved shortly after formation, were observed. (JEOL JEM-2200 FS with double Cs correction, operated at 200 KV in STEM mode. Movie playback is increased by a factor of 25). Courtesy of Andreas Verch and Roland Kroeger. For more information on Poseidon Select, go to our website at http://www.protochips.com/products/poseidon.html

Poseidon Select Liquid™ In Situ Cell: Protein Mediated Calcite Formation

Electron beam-induced growth of calcite nanoparticles in situ in the presence of AP7, a nacre protein that is found in abalone shellfish. The calcite particles were grown in the spatially confined conditions of the in-situ liquid cell in a liquid layer of 500 nm. During growth both a stable protein mediated scaffold-type structure, and an unstable crystalline structure which re-dissolved shortly after formation, were observed. (JEOL JEM-2200 FS with double Cs correction, operated at 200 KV in STEM mode. Movie playback is increased by a factor of 25). Courtesy of Andreas Verch and Roland Kroeger. For more information on Poseidon Select, go to our website at http://www.protochips.com/products/poseidon.html

Learn about Protochips' groundbreaking liquid cell for in situ TEM and STEM.

Introduction to Poseidon Select In Situ TEM Liquid Cell

Learn about Protochips' groundbreaking liquid cell for in situ TEM and STEM.

AXON Synchronicity enables live drift correction of vesicular structures in fabric softener. Data was acquired using a Gatan 626 cryogenic holder on a Tecnai G2 Twin at the Shared Materials Instrumentation Facility at Duke University. This movie demonstrates the power of AXON’s image stabilization even at cryogenic temperatures with a non-Protochips holder. For more information about AXON Synchronicity, visit us at https://www.protochips.com/solutions/tem-data-correlation/tem-software/.

Additional information: movie was roughly 10min and acceleration voltage was 200kV

Cryo Transmission Electron Microscopy with AXON Synchronicity

AXON Synchronicity enables live drift correction of vesicular structures in fabric softener. Data was acquired using a Gatan 626 cryogenic holder on a Tecnai G2 Twin at the Shared Materials Instrumentation Facility at Duke University. This movie demonstrates the power of AXON’s image stabilization even at cryogenic temperatures with a non-Protochips holder. For more information about AXON Synchronicity, visit us at https://www.protochips.com/solutions/tem-data-correlation/tem-software/.

Additional information: movie was roughly 10min and acceleration voltage was 200kV

#FindYourBreakthrough | FLASH TALKS: EP #4
In situ monitoring of exopolymer-dependent Mn mineralization on bacterial surfaces

Presented by: Dr. Thaïs Couasnon and Dr. Damien Alloyeau from Université de Paris, CNRS. Read the full paper here: https://advances.sciencemag.org/content/6/27/eaaz3125#BIBL

For more product information, visit our website at www.protochips.com or reach out to us at https://www.protochips.com/contact-us/

FLASH TALKS: EP #4 In situ Monitoring of Exopolymer dependent Mn Mineralization

#FindYourBreakthrough | FLASH TALKS: EP #4
In situ monitoring of exopolymer-dependent Mn mineralization on bacterial surfaces

Presented by: Dr. Thaïs Couasnon and Dr. Damien Alloyeau from Université de Paris, CNRS. Read the full paper here: https://advances.sciencemag.org/content/6/27/eaaz3125#BIBL

For more product information, visit our website at www.protochips.com or reach out to us at https://www.protochips.com/contact-us/

#FindYourBreakthrough | FLASH TALKS: EP #11

Monitoring of CaCO3 Nanoscale Structuration through Real-Time Liquid Phase Transmission Electron Microscopy and Hyperpolarized NMR

Presented by: Dr. Vinavadini Ramnarain from IPCMS/University of Strasbourg. Read the full publication here: https://pubs.acs.org/doi/10.1021/jacs.2c05731?ref=pdf

FLASH TALKS: EP #11 Monitoring of CaCO3 Nanoscale Structuration through Real Time Liquid Phase

#FindYourBreakthrough | FLASH TALKS: EP #11

Monitoring of CaCO3 Nanoscale Structuration through Real-Time Liquid Phase Transmission Electron Microscopy and Hyperpolarized NMR

Presented by: Dr. Vinavadini Ramnarain from IPCMS/University of Strasbourg. Read the full publication here: https://pubs.acs.org/doi/10.1021/jacs.2c05731?ref=pdf

Poseidon Select Tilt

This movie shows a series of images that demonstrates the tilt range of Poseidon Select. Images of aggregated 30 nm gold nanoparticles in 1.5 μm of water were collected in 5° increments, over a total angle of -30° to +30°. Data was collected using a Philips/FEI CM300FEG TEM equipped with a GIF energy filter at 300 kV.

Poseidon Select Liquid™ In Situ Cell: Protein Mediated Calcite Formation

Electron beam-induced growth of calcite nanoparticles in situ in the presence of AP7, a nacre protein that is found in abalone shellfish. The calcite particles were grown in the spatially confined conditions of the in-situ liquid cell in a liquid layer of 500 nm. During growth both a stable protein mediated scaffold-type structure, and an unstable crystalline structure which re-dissolved shortly after formation, were observed. (JEOL JEM-2200 FS with double Cs correction, operated at 200 KV in STEM mode. Movie playback is increased by a factor of 25). Courtesy of Andreas Verch and Roland Kroeger. For more information on Poseidon Select, go to our website at http://www.protochips.com/products/poseidon.html

Introduction to Poseidon Select In Situ TEM Liquid Cell

Learn about Protochips' groundbreaking liquid cell for in situ TEM and STEM.

Cryo Transmission Electron Microscopy with AXON Synchronicity

AXON Synchronicity enables live drift correction of vesicular structures in fabric softener. Data was acquired using a Gatan 626 cryogenic holder on a Tecnai G2 Twin at the Shared Materials Instrumentation Facility at Duke University. This movie demonstrates the power of AXON’s image stabilization even at cryogenic temperatures with a non-Protochips holder. For more information about AXON Synchronicity, visit us at https://www.protochips.com/solutions/tem-data-correlation/tem-software/.

Additional information: movie was roughly 10min and acceleration voltage was 200kV

FLASH TALKS: EP #4 In situ Monitoring of Exopolymer dependent Mn Mineralization

#FindYourBreakthrough | FLASH TALKS: EP #4
In situ monitoring of exopolymer-dependent Mn mineralization on bacterial surfaces

Presented by: Dr. Thaïs Couasnon and Dr. Damien Alloyeau from Université de Paris, CNRS. Read the full paper here: https://advances.sciencemag.org/content/6/27/eaaz3125#BIBL

For more product information, visit our website at www.protochips.com or reach out to us at https://www.protochips.com/contact-us/

FLASH TALKS: EP #11 Monitoring of CaCO3 Nanoscale Structuration through Real Time Liquid Phase

#FindYourBreakthrough | FLASH TALKS: EP #11

Monitoring of CaCO3 Nanoscale Structuration through Real-Time Liquid Phase Transmission Electron Microscopy and Hyperpolarized NMR

Presented by: Dr. Vinavadini Ramnarain from IPCMS/University of Strasbourg. Read the full publication here: https://pubs.acs.org/doi/10.1021/jacs.2c05731?ref=pdf

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